Inactivation of Ricin using 4-azidophenyl-beta-D-galactopyranoside and 4-diazophenyl-beta-D-galactopyranoside.
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چکیده
منابع مشابه
Disorder and conformational analysis of methyl [beta]-d-galactopyranosyl-(1-->4)-[beta]-d-xylopyranoside
Methyl -d-galactopyranosyl-(1!4)-d-xylopyranoside, C12H22O10, (II), crystallizes as colorless needles from water with positional disorder in the xylopyranosyl (Xyl) ring and no water molecules in the unit cell. The internal glycosidic linkage conformation in (II) is characterized by a ’0 torsion angle (C2Gal—C1 0 Gal—O1 0 Gal—C4Xyl) of 156.4 (5) and a 0 torsion angle (C1Gal—O1 0 Gal—C4Xyl—C3Xyl...
متن کاملUtilization and metabolism of [U-14C]4' galactosyllactose (O-beta-D-galactopyranosyl-(1----4)-O-beta-D-galactopyranosyl-(1----4)- D-glucopyranose) in rats.
O-beta-D-Galactopyranosyl-(1----4)-O-beta-D-galactopyranosyl- (1----4)-D-glucopyranose (designated as 4'GL) are produced from lactose with Cryptococcus laurentii OKN-4. Excretion and metabolism of 4'GL in rats were examined using a radioisotope technique. [U-14c]4'GL was synthesized from [U-14C]lactose by Cryptococcus laurentii OKN-4. The 14CO2 in expired air was counted after oral administrati...
متن کاملUse of fluorescein-di-beta-D-galactopyranoside (FDG) and C12-FDG as substrates for beta-galactosidase detection by flow cytometry in animal, bacterial, and yeast cells.
Fluorescein-di-beta-D-galactopyranoside (FDG) was found to be a useful substrate for beta-galactosidase detection by flow cytometry in gram-negative bacteria, since it entered viable cells and gave a fluorescence emission proportional to the enzymatic activity. C12-FDG, a more lipophilic derivative, gave a very poor signal because of the lack of penetration. On the contrary, C12-FDG was more se...
متن کاملphthalein /3-D-glucuronide, p-nitrophenyl N-acetyl-/3-D-glucosaminide, p-nitrophenyl /3-D-fucoside, p- nitrophenyl /3-D-galactoside and 6-bromo-2-naphthyl /3-D-galactopyranoside from Sigma Chemical CO.(U.S.A..), phenyl /3-D-galactopyranoside from Nakarai
p-Hydroxyphenylacetaldoxime (HPAAO) (anti and syn forms) obtained by fermentation arid its analogs chemically synthesized were tested for their activities to inhibit various glycosidases. HPAAO inhibited bovine liver /3-galactosidase in a competitive manner at pH 17.0 with an apparent Ki value of 8 x 10-8 M. HPAAO also inhibited various mammalian /3.glycosidases which had pH optima between 6.0 ...
متن کاملSoluble uridine diphosphate D-galactose: D-glucose beta-4-D-galactosyltransferase from bovine milk.
Some of the properties of a 70-fold purified bovine milk lactose synthetase have been determined. The enzyme preparation appears to be specific for uracil nucleoside diphosphate D-galactose derivatives. Adenosine, thymidine, @dine, or guanosine diphosphate derivatives cannot serve as galactosyl donors for n-glucose to form lactose. The enzyme preparation will not transfer the galactosyl residue...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1983
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)32353-6